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KMID : 0903619940350050449
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Journal of the Korean Society for Horticultural Science
1994 Volume.35 No. 5 p.449 ~ p.456
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Identification of Cucurbitaceae Cultivars by Using RAPD Markers
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김병동/Kim, Byung Dong
엄영현/빈철구/홍규현/전홍정/Om, Young Hyun/Been, Chul Gu/Hong, Kue Hyon/Jeon, Hong Jeong
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Abstract
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Random amplified polymorphic DNA (RAPD) markers generated by 6 out of 50 arbitrary 10-mer primers were effective in discrimination among 9 Cucurbita moschata and 6 C. pepo cultivars. The 6 primers produced 64 useful RAPD markers, and their sizes ranged from 350 to 6,000 base pairs. Eleven percent of the markers were common in both C. moschata and C. pepo, whereas 62.5% were specific to either species. The rest were polymorphic in either or both species. The average dissimilarity coefficient matrix of markers was 5.84 between C. moschata and C. pepo markers, 3.41 between two C. moschata cultivars and 2.90 between two C. pepo cultivars. Fifteen Cucurbita cultivars screened here were distinguished from each other by a single or by combinations of 2 to 3 primers.
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